miRkwood small RNA-seq

Job title (optional)

Upload your set of reads

This must be a BED file created by our script mirkwood-bam2bed.pl [?]

Select an assembly

Select a reference genome in the list below [?]

Parameters

Mask coding regions [?]

Filter out tRNA/rRNA/snoRNA [?]

Remove multiply mapped reads ( > 5 positions) [?]

Select only sequences with MFEI < -0.6 [?]

Compute thermodynamic stability (shuffled sequences) [?]

Flag conserved mature miRNAs (alignment with miRBase + miRdup) [?]

Filter out low quality hairpins [?]

E-mail address (optional)