Enter query sequence: Paste your sequence(s) in FASTA format [?]
or, upload a file
Scan both strands [?]
Mask coding regions (BlastX) [?]
Filter out tRNA/rRNA (tRNAscan-SE / RNAmmer) [?]
clear | run with an example
Parameters: Choose the annotation criteria for the miRNA precursors [?]
Select only sequences with MFEI < -0.6
Compute thermodynamic stability (shuffled sequences)
Flag conserved mature miRNAs (alignment with miRBase + miRdup)